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Maria Syldona,
Ph.D.
Quantum Biology
Research Labs
PO Box 157
Northport, NY 11768
Summary
Electromagnetic (EM) fields
from cell phones were shown to have a statistically significant detrimental effect
on the recovery of human DNA after heat shock. This effect was observed with
cell phones on standby mode when they are emitting relatively weak EM fields
and after only minutes of EM field exposure. The detrimental effect of EM
energy from cell phones was completely neutralized when Aulterras Neutralizer
was attached to the back of the cell phone. In the presence of the Neutralizer
there was 100% recovery of the DNA, as if no cell phone was present. This
neutralizing effect appears to prevent initial damage to DNA immediately following
EM field exposure.
The
Experimental Approach
In these experiments the
biological system being influenced by the EM fields from cell phones is purified
human DNA suspended in a natural ionic environment. Previous studies demonstrated
that the structure of DNA is sensitive to classical (Semin, 1995) and non-classical
(Rein, 1995) EM fields, thereby indicating that energy fields can influence the
winding and unwinding of the two strands which make up the DNA helix and define
its conformation.
Utilizing the well known
fact that heat shock causes DNA strands to unwind (Marmur, 1961), the Quantum
Biology Research Lab developed a sensitive assay which involves measuring the
kinetics of rewinding following heat shock. It is well known that as the DNA
cools, it recovers by rewinding back into an intact double helix (Marmur, 1961).
The rewinding/ recovery process can be monitored by measuring the absorption
of UV light as a function of the cooling temperature or increasing time. As the
DNA recovers from the heat shock, hydrogen bonds reform to connect the two strands.
Thus healing or recovery of the DNA is directly related to the number of hydrogen
bonds. Preliminary experiments with the new assay confirmed that weak EM fields
slow down the rewinding or recovery following heat shock.
More recently the DNA rewinding
assay has been modified to increase its sensitivity. The modifications are based
on a previous study which demonstrated that the sensitivity of DNA to UV light
is enhanced in the presence of small amounts of iron (ferric ions) (Audic, 1993).
The modified assay, which utilizes trace amounts of iron, has several advantages
over the original assay. Using the modified assay, weak EM field effects, reported
in this study, were observed within the first few minutes following EM exposure
and did not require conditioning (repeated exposure to EM fields).
Experimental
Methods
Three types of experiments
were conducted in this study. The control experiments were done first in the
presence of ambient EM fields, but in the absence of any man-made EM fields.
In the electromagnetic experiments, DNA recovery was measured in the presence
of EM fields from cell phones. The third set of experiments involved measuring
DNA recovery in the presence of neutralized EM fields from cell phones containing
the Neutralizer.
The specific protocol that
was followed involved making a stock solution (0.4mg/ml) of human placental DNA
(Sigma Chemical Co., St. Louis) in deionized water. The stock solution was diluted
to 0.03mg/ml in 5mM NaCl containing 1-4 µM FeCl and heat shocked (80° C
for 5 minutes). A concentration of 1 µM FeCl was shown to be optimal and
used in the final experiments. Immediately after heat treatment the DNA was gently
transferred to a quartz cuvette and then placed in the cuvette holder inside
the spectrophotometer. For EM field exposure a mobile Nokia cell phone (plugged
in and set on standby mode) was placed face up on top of the cuvette. To neutralize
the EM fields, the Neutralizer was placed on the back of the cell phone directly
below the antennae. The cell phone containing the Neutralizer was then placed
face up on top of the cuvette. In these experiments normal and neutralized cell
phones were identically placed on the cuvette immediately after heat treatment
and remained there for the duration of the experiment. The same protocol was
used for these two sets of experiments, as well as for controls, which were done
in the absence of any cell phone treatment.
For all experiments, the
conformation of DNA was measured with state-of-the art biochemical methodology
using a UV-visible diode array spectrophotometer (Hewlett Packard 8451A) to quantify
the amount of UV light absorbed (Thomas, 1995). UV measurements began immediately
after the cell phones were placed on the cuvette. Absorption of UV light at 260nm
was measured every 60 seconds over the course of one hour following heat shock.
As the DNA recovers, it naturally rewinds and the absorption of light gradually
decreases. The slope of the rewinding curve is a measure of DNAs ability
to recover after heat shock. In order to quantify the speed of recovery, the
slopes were calculated using IBM Excel software.
| |
Average
Slope |
SD |
n |
p
(wrt control) |
Control |
0.877 |
0.041 |
6 |
|
Cell Phone |
0.687 |
0.189 |
8 |
0.02 |
Cell Phone +
Neutralizer |
0.872 |
0.063 |
6 |
NS |
RESULTS
For the data presented
in Figure 1 only the initial portion (the first five minutes) of the entire recovery
curve was analyzed. The slope in this region corresponds to the initial recovery
rate, classically used by biochemists in studying enzyme kinetics. The light
grey irregular line in this figure is a plot of the raw absorption data collected
by the spectrophotometer as a function of time after heat shock. The solid black
line is the computer generated best-fit calculation of the slope (r2). The slope
was calculated for each separate experiment and then compared statistically using
a two sample t-test (assuming equal variance). For statistical analyses a total
of 6 control experiments, 8 EM experiments and 6 neutralized EM experiments were
used.
A.
Electromagnetic Fields from Cell Phones Effect DNA Recovery
The results demonstrate
the effect of electromagnetic (EM) fields from cell phones on DNA recovery after
heat shock. In the absence of EM fields (control experiments) a negative slope
for DNA recovery is obtained. A less negative value for the slope reflects a
slower recovery following heat shock. In the absence of EM fields the average
slope (recovery) was -0.877 ± 0.041. In the presence of EM fields from
the cell phone the slope had an average value of -0.687 ± 0.189. This
indicates that the EM field from the cell phone produced a 22% inhibition of
recovery. This detrimental effect of EM fields from the cell phone is highly
significant compared to the untreated control (p<0.02).
B.
Cell Phone Damage Reversed with the Neutralizer
The cell phone containing
the Neutralizer produced a similar effect on DNA recovery as was observed in
the controls. The average slope for these experiments was - 0.872 ± 0.063.
This result is not significantly different than the control value indicating
that the harmful effect of the EM field from the cell phone is completely neutralized
by the presence of the Neutralizer.
Discussion
A previous study from the
Quantum Biology Research Lab (Syldona, 2000) demonstrated an unusual effect when
a test tube containing human DNA in an aqueous solution was physically placed
on top of the Aulterra powder, the active ingredient of the Neutralizer. The
results demonstrated that the powder radiates an energy field which resonates
with DNA producing an oscillatory winding and unwinding behavior in its secondary
structure. Such behavior is consistent with the newly discovered phenomenon in
biological systems referred to as macroscopic quantum coherence (Chudnovsky,
2000) and suggests that the energy radiating from the Aulterra powder is highly
coherent (laser-like). Since the dielectric material in the powder will radiate
classic (incoherent) EM energy, it is conceivable that the activation procedure
in the manufacturing of the powder makes this intrinsic energy coherent. Alternatively,
the unique mixture of minerals in the Aulterra powder may have intrinsic quantum
properties, like other complex lattice structures which also exhibits quantum
properties (Orrit, 1986).
The ability of Aulterras
powder to radiate quantum fields is related to its ability to store quantum information
following activation. Quantum information storage is now a recognized technology
in the computer industry which involves optical and magneto-optical storage.
Information storage in computers and in Aulterras products share a common
mechanism, since they both require an external EM field to store the information
and both result in the emission of a different type of EM field. Some variations
of computer technology allow storage of information in three dimensional lattice
structures (Mok, 1991), similar to Aulterras mineral base. In other variations
of computer technology, the emitted EM field can be a coherent laser (Reif, 1991).
Furthermore, certain storage media exhibit unusual anomalous behavior referred
to as space-inversion symmetry (ie. normal symmetry associated with magnetization
of the optical materials is broken) (Pavlov, 1997). A similar situation also
exists in phase conjugating systems, where a time-reversed longitudinal wave
is emitted (Zozulya, 1994). According to quantum physics, both of these situations
occur at the quantum level and are associated with the presence of quantum fields
(Blumel, 1992). Therefore, if these established systems are capable of emitting
a quantum field, Aulterras technology may also do the same.
If the Aulterra powder
radiates a highly coherent field, how could such a field neutralize the harmful,
incoherent radiation emitted from a cell phone? Although traditional physics
theory states that two force fields dont interact in space (they just pass
through each other), recent experiments indicate that such interactions are possible.
Working with two incoherent light sources, Comorosan demonstrated that one could
modulate the action of the other to modulate a chemical reaction (Comorosan,
1990). In addition, Omura demonstrated that energy associated with a chemical
enzyme inhibitor, in powder form, could produce a biological effect when the
molecular energy was used to modulate a laser (Omura, 1994). In this sense the
coherent laser energy acted as a carrier for the molecular energy. These studies
demonstrate that two energy fields can in fact interact. Furthermore, mixing
incoherent and coherent energies can have differential effects on biological
systems when the ratio of incoherent to coherent energies is altered (Litovitz,
1994).
These studies offer an
explanation for the ability of a coherent field generated from the Aulterra powder
to neutralize the biological effects of incoherent energy from a cell phone.
These studies also explain the results in the previous study by the Quantum Biology
Research Lab (Syldona, 2000). Coherent energy from the Aulterra powder could
therefore neutralize the detrimental biological effect of the molecular energy
associated with heavy metals. In these experiments, however, the biological system
(DNA) was being influenced by both the energy from the chemical toxin and the
chemical toxin itself. Nonethess, the Aulterra product offered complete protection.
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